Abstract:Objective To explore the expression of serum microRNA (miR)-335 and the latent transforming growth factor (TGF) -β binding protein 2 (LTBP-2) in patients with coronary heart disease (CHD) and the clinical significance.? Methods A total of 96 patients with CHD from February 2014 to February 2017 including 34 stable angina pectoris (SAP) patients, 32 unstable angina pectoris (UAP) patients and 30 acute myocardial infarction (AMI) patients were selected as research objects, and 30 healthy subjects of physical examination at the same time were selected as control group. Real time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression level of serum miR-335. Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of serum LTBP-2.? Results With the severity of CHD(according to health check-up, SAP, UAP, AMI order) gradually increased, the expression level of serum miR-335 decreased gradually (P<0.01), while the level of serum LTBP-2 increased (P<0.01), and there were statistical differences between any two adjacent grades (all P<0.05). There were a significant negative correlation between the expression level of serum miR-335 and the Gensini score (r=-0.532, P＜0.05) and a significant positive correlation between the level of serum LTBP-2 and the Gensini score (r=0.796, P＜0.05) and a significant negative correlation between serum miR-335 expression level and serum LTBP-2 content (r=-0.547, P＜0.05).? Conclusion The miR-335 presents low expression in serum for CHD patients, and serum LTBP-2 level increases significantly, and both them are expected to be the reference indexes for predicting the severity of CHD disease.