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投稿时间：2019-10-15 网络发布日期：2020-06-20
投稿时间：2019-10-15 网络发布日期：2020-06-20
中文摘要: 目的 探讨黄芪甲苷对卵巢癌SKOV3细胞增殖、迁移侵袭能力的影响,并初步探讨其机制。方法 选取人卵巢癌SKOV3细胞,以黄芪甲苷浓度为0、0.2、0.4、0.8、1.6、3.2 mg/ml分别作用于细胞,采用CCK-8法及克隆形成实验探讨黄芪甲苷对SKOV3细胞增殖能力的影响;采用Transwell细胞迁移和侵袭实验观察黄芪甲苷对SKOV3细胞迁移、侵袭能力的影响;采用Western Blot法检测黄芪甲苷处理后的SKOV3细胞肿瘤转移相关蛋白基质金属蛋白酶(MMP)2、MMP9的表达。结果 0.2 mg/ml浓度以上的黄芪甲苷显著抑制卵巢癌SKOV3细胞的增殖能力,并存在浓度和时间依赖性;Transwell迁移实验结果显示,0 mg/ml黄芪甲苷组穿过小室细胞数为97.20±4.66,0.4 mg/ml黄芪甲苷组穿过小室的细胞数为47.60±5.41,明显少于0 mg/ml黄芪甲苷组(P<0.01)。Transwell侵袭实验结果显示,0 mg/ml黄芪甲苷组穿过小室细胞数为74.20±5.45,0.4 mg/ml黄芪甲苷组穿过小室的细胞数为33.20±4.32,明显少于0 mg/ml黄芪甲苷组(P<0.01)。经0.4 mg/ml黄芪甲苷处理后SKOV3细胞表现出不同程度抑制MMP2、MMP9蛋白表达的现象。结论 黄芪甲苷可明显抑制卵巢癌SKOV3细胞的体外增殖和迁移、侵袭能力,这可能与其下调MMP2、MMP9蛋白的表达有关。
Abstract:Objecitve To investigate the effect of astragaloside on the proliferation, migration and invasion of ovarian cancer cell SKOV3 and its mechanism. Methods Human ovarian cancer cell SKOV3 was selected and treated with astragaloside concentration of 0, 0.2, 0.4, 0.8, 1.6 and 3.2 mg/ml respectively.The effect of astragaloside on the proliferation of SKOV3 was studied by CCK-8 method and clonal formation experiment.Transwell cell migration and invasion experiment were used to observe the effect of astragaloside on the migration and invasion of SKOV3.Western blot was used to detect the expression of matrix metalloproteinase (MMP) 2 and MMP 9 in SKOV3 treated with astragaloside. Results Astragaloside at a concentration of more than 0.2 mg/ml significantly inhibited the proliferation of SKOV3 in a concentration- and time-dependent manner.Transwell migration showed that the number of cells passing through the chamber in the 0.4 mg/ml astragaloside group was significantly less than that in 0 mg/ml astragaloside group (47.60±5.41 vs 97.20±4.66, P<0.01).The results of Transwell invasion experiment showed that the number of cells passing through the chamber in 0.4 mg/ml astragaloside group was significantly less than that in 0 mg/ml astragaloside group (33.20±4.32 vs 74.20±5.45, P<0.01).After treated with 0.4 mg/ml astragaloside SKOV3 showed inhibition of MMP2 and MMP9 protein expression in varying degrees. Conclusion Astragaloside can inhibit the proliferation, migration and invasion of SKOV3 cells in vitro, which may be related to the down-regulation of MMP2 and MMP9 protein expression.
keywords: Astragaloside Ovarian cancer Proliferation Migration Invasion Matrix metalloproteinase 2 Matrix metalloproteinase 9
文章编号： 中图分类号： 文献标志码：A
基金项目:
| Author Name | Affiliation |
| CHENG Yan, REN Qian-chuan | Department of Gynecology, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China |
引用文本：
程燕,任黔川.黄芪甲苷对卵巢癌SKOV3细胞增殖与迁移侵袭作用机制[J].中国临床研究,2020,33(6):743-748.
程燕,任黔川.黄芪甲苷对卵巢癌SKOV3细胞增殖与迁移侵袭作用机制[J].中国临床研究,2020,33(6):743-748.