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投稿时间：2018-09-06 网络发布日期：2018-12-20
投稿时间：2018-09-06 网络发布日期：2018-12-20
中文摘要: 目的 探讨不同浓度钛合金颗粒对小鼠成骨细胞核因子-κB受体活化因子配基(RANKL)及骨保护素(OPG)表达的影响,以及细胞外信号调控激酶1/2(ERK1/2)信号通路在其中的作用。 方法 体外培养小鼠成骨细胞,按照培养基中是否加用ERK1/2特异性抑制剂(PD98059),将成骨细胞分为无PD98059干预组和PD98059干预组两个大组,每大组又分为空白对照组,钛合金颗粒干预A组(0.01 g/L)、B组(0.1 g/L)和C组(1.0 g/L),每组6个样本。分别在培养第24 h和48 h两个时间点,收集各组细胞,采用逆转录聚合酶链反应（RT-PCR）检测成骨细胞内RANKL和OPG mRNA的相对表达量。 结果 RT-PCR结果显示,与不同浓度钛颗粒共培养24 h、48 h后,各组成骨细胞均有RANKL、OPG mRNA的表达;在各时间点A组、B组和C组成骨细胞RANKL和OPG mRNA相对表达量以及RANKL/OPG mRNA比值均较对照组显著增加(P<0.05,P<0.01)。PD98059干预组成骨细胞RANKL、OPG mRNA相对表达量以及RANKL/OPG mRNA比值均低于无PD98059干预组(P<0.01,P<0.05)。 结论 不同浓度钛合金颗粒可促进成骨细胞RANKL mRNA、OPG mRNA的表达,尤其是RANKL mRNA的表达,同时也能上调RANKL/OPG的比值。ERK信号通路抑制剂PD98059可一定程度抑制OPG的表达,尤其是RANKL的表达,提示ERK1/2信号通路可能参与调节钛合金颗粒诱导的成骨细胞RANKL、OPG的表达。
中文关键词: 成骨细胞 钛合金颗粒 核因子-κB受体活化因子配基 骨保护素 细胞外信号调控激酶1/2信号通路
Abstract:Objective To investigate the effects of different concentrations of titanium alloy particles on the expressions of osteoprotegerin (OPG) and receptor activator of nuclear factor (NF) -κB ligand (RANKL) in mouse osteoblasts and the role of extracellular signal-regulated kinase-1 and-2 (ERK1/2) signaling pathway. Methods The mouse osteoblasts were cultured in vitro. According to whether the PD98059, an ERK1/2 inhibitor to be added in medium, the osteoblasts were divided into no PD98059 intervention group and PD98059 intervention group, and both of them were re-divided into blank control group, intervention groups of different concentrations of titanium alloy particles: group A (0.01 g/L), group B (0.1 g/L) and group C (1.0 g/L). Six samples were taken in each group. The cells of each group were collected at the time points of 24-hour and 48-hour after culture, and the expressions of RANKL mRNA and OPG mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR). Results RT-PCR showed that the expressions of RANKL mRNA and OPG mRNA were found in osteoblasts of all groups after co-culture with different concentrations of titanium particles for 24 hours and 48 hours, and the expressions of RANKL mRNA and OPG mRNA in osteoblasts and the ratio of RANKL mRNA to OPG mRNA at each time point increased significantly in groups A, B, C compared with blank control group (all P<0.01). The expressions of RANKL mRNA and OPG mRNA in osteoblasts and the ratio of RANKL mRNA to OPG mRNA in PD98059 intervention group were all lower than those in no PD98059 intervention group (P<0.01, P<0.05). Conclusion Titanium alloy particles of different concentrations can promote the expressions of RANKL mRNA and OPG mRNA in osteoblasts, especially the expression of RANKL mRNA and can also up-regulate the ratio of RANKL mRNA to OPG mRNA. ERK signaling pathway inhibitor PD98059 can inhibit the expressions of RANKL mRNA and OPG mRNA to some extent, especially the expression of RANKL mRNA. It is suggested that ERK1/2 signaling pathway is involved in regulating the expressions of RANKL and OPG in osteoblasts induced by titanium alloy particles.
keywords: Osteoblast Titanium alloy particles Receptor activator of nuclear factor-κB ligand Osteoprotegerin Extracellular signal-regulated kinase1/2 signaling pathway
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基金项目:
| 作者 | 单位 |
| 金清1 | 1.南京医科大学附属淮安第一医院手术麻醉科,江苏 淮安 223300 |
| 谢守祥2 | 2.南京医科大学附属淮安第一医院急诊科,江苏 淮安 223300 |
| 许铁3 | 3.徐州医科大学附属医院急救中心,江苏 徐州 221002 |
| 孙虹2 | 2.南京医科大学附属淮安第一医院急诊科,江苏 淮安 223300 |
| 严庄2 | 2.南京医科大学附属淮安第一医院急诊科,江苏 淮安 223300 |
引用文本：
金清,谢守祥,许铁,等.ERK信号通路对不同浓度钛合金颗粒诱导成骨细胞RANKL和OPG表达的影响[J].中国临床研究,2018,31(12):1614-1618.
金清,谢守祥,许铁,等.ERK信号通路对不同浓度钛合金颗粒诱导成骨细胞RANKL和OPG表达的影响[J].中国临床研究,2018,31(12):1614-1618.