Abstract:Objective To explore the influences of Vinculin expression on the biological characteristics of non-small cell lung cancer(NSCLC) A549 cell lines through in-vitro experiments. Methods After being purchased and cultured in vitro,human NSCLC A549 cell lines were divided into control group, blank vector group(transfected with blank vector) and high expression group(transfected with Vinculin overexpression vector). After in-vitro culture for 48 hours,RT-PCR was used to detect the expression of Vinculin mRNA in A549 cells, MTT was used to measure the cell viability, Ki-67 immunofluorescence was used to determine the cell proliferation, and Transwell culture system and Hoechst staining was used to observe the migration and invasion ability of A549 cells. Results RT-PCR showed that the expression of Vinculin mRNA in high expression group significantly increased compared with control group and blank vector group (P<0.01). MTT assay showed that the OD value in high expression group was significantly lower than that in control group and blank vector group (P<0.01). Ki-67 immunofluorescence test showed that the number of Ki-67⁺ cells in high expression group significantly decreased compared with control group and blank vector group (P<0.01). Transwell culture system and Hoeschst staining results showed that the number of migrated and invaded cells in high expression group was significantly lower than those in control group and blank vector group (P<0.01). Conclusions Overexpression of Vinculin can inhibit the proliferation, migration and invasion of NSCLC A549 cell line. Vinculin may be a potential biological target for the diagnosis and treatment of NSCLC.