Abstract:Objective To investigate the noninvasive evaluation of virtual touch tissue imaging quantification (VTIQ) in the rat model of hepatic sinusoidal obstruction syndrome (HSOS) induced by monocrotaline. Methods A total of 70 SD rats were randomly divided into control group (n=10) and experimental group (n=60).Rats in the control group were given 2 ml tap-water by gavage.Rats in the experimental group were given 2ml monocrotaline(130 mg/kg) by gavage.Rats in the MCT group (n=30) were only given monocrotaline by gavage.Rats in the MCT-L group (n=30) were given low molecular weight heparin (96 iu,bid) by subcutaneous injection 24 hours after monocrotaline gavage.The portal vein diameter and blood flow velocity were detected by conventional ultrasonography on the 3rd and 5th day,and then the shear wave velocity (SWV) of liver was measured by VTIQ.Within 2 hours after the VTIQ,15 rats in MCT group and 15 rats in MCT-L group were killed.Liver samples were taken for pathological examination,and the lesions were determined according to the Deleve grading standard under light microscopy. Results The body weight of rats decreased after a single intragastric administration of monocrotaline.Compared with the control group,the inner diameter of portal vein in MCT group and MCT-L group had no significant differences after 3 and 5 days′ gavage(P>0.05).The portal vein velocity decreased,the liver SWV value increased and the liver histological score increased significantly (P<0.05).After 3 and 5 days′ gavage,there was no significant difference in the diameter of portal vein between MCT-L group and MCT group(P>0.05),but the portal vein flow rate was significantly higher,liver SWV value and liver histological score were significantly lower in MCT-L group (P<0.05). Conclusion VTIQ could be used to detect SWV of liver,which could reflect the pathological changes of liver tissue and evaluate the therapeutic effect of low molecular weight heparin in the treatment of monocrotaline induced HSOS noninvasively.