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投稿时间：2020-05-26 网络发布日期：2021-01-20
投稿时间：2020-05-26 网络发布日期：2021-01-20
中文摘要: 目的 探讨内质网相关降解蛋白Derlin-1在非小细胞肺癌(NSCLC)中的表达及其与NSCLC临床特征和预后的相关性。
方法 回顾性分析2010年1月至2011年12月在南通大学附属医院手术治疗确诊的40例NSCLC患者的临床资料。按TNM分期分为Ⅰ～Ⅱ期22例,Ⅲ～Ⅳ期18例,其中18例患者癌旁组织作对照组。应用实时PCR、Western blot、免疫组化技术检测癌组织和癌旁组织中Derlin-1的表达,分析其表达与NSCLC临床特征及预后的关联性,分析Derlin-1阳性与NSCLC患者3年总生存率的关系。
结果 Derlin-1的mRNA和蛋白相对表达量,呈Ⅲ～Ⅳ期癌组织>Ⅰ～Ⅱ期癌组织>癌旁组织,总体及两两比较差异均有统计学意义(P<0.01，P<0.05)。Ⅲ~Ⅳ期、Ⅰ~Ⅱ期和对照组Derlin-1阳性率分别为94.44%、63.64%和16.67%,三组间差异有统计学意义(P<0.01);Derlin-1阳性率与NSCLC的TNM分期、病理分级、淋巴结转移有关联性（P<0.05，P<0.01）;Derlin-1阴性NSCLC患者3年生存率明显高于Derlin-1阳性患者(77.78% vs 22.58%,P<0.01)。
结论 Derlin-1在NSCLC中过表达,表达高者恶性程度高,阳性表达者预后差，Derlin-1或可作为NSCLC治疗的潜在靶点。
中文关键词: 非小细胞肺癌 内质网相关的降解蛋白Derlin-1 预后 临床特征
Abstract:Objective To investigate the expression of endoplasmic reticulum associated degradation protein Derlin-1 in non-small cell lung cancer (NSCLC) tissues and explore its associations with clinical features and prognosis in NSCLC patients.
Methods A retrospective analysis was performed on 40 patients with histologically confirmed NSCLC from January 2010 to December 2011 in Affiliated Hospital of Nantong University.According to TNM classifications,the patients were divided into Ⅰ-Ⅱ stage group (n=22) and Ⅲ-Ⅳ stage group (n=18).The normal tissues adjacent to cancer of 18 subjects in all patients were used as control group.Real-time PCR,Western blot and immunohistochemistry were respectively used to detect the expressions of Derlin-1 in cancer tissues and adjacent tissues.The associations of Derlin-1 expression with clinical characteristics and prognosis of NSCLC and the relationship between positive expression of Derlin-1 and overall 3-year survival rate were analyzed.
Results The relative expressions of Dertlin-1 mRNA and protein decreased significantly in the order of Ⅲ-Ⅳ group,Ⅰ-Ⅱ group and control group,overall and pairwis comparison were statistic significant (P<0.01,P<0.05).The positive rates of Derlin-1 in Ⅲ-Ⅳ group，Ⅰ-Ⅱ group and control group were 94.44%,63.64% and 16.67%,respectively,and there were statistical differences in them among three groups（P＜0.01）.The positive rate of Derlin-1 was statistically correlated with TNM stage,pathological grading and lymph node metastasis(P<0.05,P<0.01);the 3-year overall survival rate in patients with positive expression of Derlin-1 was significantly lower than that in patients with negative expression of Derlin-1 (77.78% vs 22.58%,P＜0.01).
Conclusion
Derlin-1 is overexpressed in NSCLC and results in high malignancy and poor prognosis.Derlin-1 might be served as a potential target for the treatment of NSCLC.
keywords: Non-small cell lung cancer Endoplasmic reticulum associated degradation protein Derlin-1 Prognosis Clinical features
文章编号： 中图分类号：R734.2 文献标志码：A
基金项目:南通市通州区民生和社会事业类科技计划项目(通科[BFB]［2016］38-1)
| Author Name | Affiliation |
| CHENG Wei，GE Shan-shan,LU Bin,CAO Yu-hua | Department of Respiratory,Nantong Tongzhou People′s Hospital,Nantong,Jiangsu 226300，China |
引用文本：
成炜, 葛珊珊, 陆彬, 曹玉华.Derlin-1的表达与非小细胞肺癌临床特征及预后的相关性[J].中国临床研究,2021,34(1):23-26.
成炜, 葛珊珊, 陆彬, 曹玉华.Derlin-1的表达与非小细胞肺癌临床特征及预后的相关性[J].中国临床研究,2021,34(1):23-26.