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投稿时间：2020-06-11 网络发布日期：2021-01-20
投稿时间：2020-06-11 网络发布日期：2021-01-20
中文摘要: 目的 探讨长链非编码RNA(lncRNA)牛磺酸上调基因1(TUG1)与尿路上皮癌相关抗原1(UCA1)在非小细胞肺癌(NSCLC)患者血浆中的表达及诊断价值。
方法 采集2018年7月至2019年10月在江苏大学附属医院呼吸内科及胸外科收治的60例NSCLC及60例良性肺病患者血浆标本,采用实时荧光定量聚合酶链反应(qRT-PCR)方法检测lncRNA TUG1与lncRNA UCA1在两组对象血浆中的相对表达量;分析lncRNAs的表达水平与NSCLC患者临床病理特征的关系;构建受试者工作特征(ROC)曲线,分析血浆TUG1、UCA1的表达水平对NSCLC的诊断效能,同时与经典的肿瘤标志物癌胚抗原(CEA)、细胞角蛋白19(CYFRA21-1)进行比较。
结果 与良性肺病组相比,NSCLC组中血浆TUG1表达下调,UCA1表达上调(P均<0.01)。血浆TUG1与UCA1的高低表达在NSCLC患者的性别、年龄、吸烟史、肿瘤直径、病理分型、TNM分期、淋巴结转移、远处转移方面差异无统计学意义(P均>0.05)。ROC曲线分析表明,单独诊断NSCLC时,TUG1的ROC曲线下面积(AUC=0.72)与CEA的AUC(0.77)相近,大于CYFRA21-1的AUC(0.66),而UCA1的AUC(0.69)与CYFRA21-1相近。TUG1、UCA1的诊断敏感性(81.67%、83.33%)略高于CEA、CYFRA21-1(58.33%、70.00%)。与单独诊断相比,TUG1与UCA1联合诊断NSCLC的AUC最高(0.82),在敏感性较好保持的情况下,特异性(70.00%)有所提升。
结论 与良性肺病患者相比，NSCLC患者血浆TUG1表达下调，UCA1表达上调，
TUG1与UCA1联合诊断NSCLC优于TUG1、UCAI、CEA、CYFRA21-1四指标单独诊断,有望成为诊断NSCLC的潜在生物标志物。
中文关键词: 非小细胞肺癌 血浆 长链非编码核糖核酸 牛磺酸上调基因1 尿路上皮癌相关抗原1
Abstract:Objective To investigate the plasma expression levels and diagnostic value of long non-coding RNA (lncRNA) taurine-upregulated gene 1 (TUG1) and lncRNA urothelial carcinoma-associated antigen 1(UCA1) in patients with non-small cell lung cancer (NSCLC).
Methods Plasma samples of 60 patients with NSCLC (NSCLC group) and 60 patients with benign lung disease(control group)from July 2018 to October 2019 were collected,and the plasma expression levels of lncRNA TUG1 and lncRNA UCA1 were detected by real-time quantitative polymerase chain reaction (qRT-PCR) to analyze the relationship between the plasma expression levels of lncRNAs and the clinicopathological characteristics.Receiver operating characteristic (ROC) curve was constructed to analyze the diagnostic efficacy of plasma TUG1 and UCA1 levels on NSCLC,which was compared with the classical tumor markers carcinoembryonic antigen(CEA) and cytokeratin 19 fragment(CYFRA21-1) at the same time.
Results Compared with control group,the plasma expression level of TUG1 was down-regulated,and the plasma expression level of UCA1 was up-regulated in NSCLC group significantly (all P<0.01).
There was no significant difference between the high and low levels of plasma TUG1 and UCA1 expression in NSCLC patients′ gender,age,smoking history,tumor diameter,pathological classification,TNM stage,lymph node metastasis and distant metastasis,etc.(all P>0.05).ROC curve showed that when diagnosing NSCLC alone,AUC of TUG1 (0.72) was close to that of CEA (0.77),but greater than that of CYFRA21-1 (0.66),and AUC of UCA1 (0.69) was similar to that of CYFRA21-1.The sensitivities of TUG1 and UCA1 were 81.67% and 83.33%,respectively and were slightly higher than those of CEA (58.33%) and CYFRA21-1(70.00%).Compared with the individual diagnosis,the AUC of combined diagnosis of NSCLC by TUG1 and UCA1 was the highest (0.82),and the specificity (70.00%) was improved when the sensitivity was well maintained.
Conclusions Compared with those in patients with benign lung disease,TUG1 is down-regulated,and UCA1 is up-regulated in plasma of NSCLC patients.The diagnosis efficiency of TUG1 combined with UCA1 for NSCLC is better than that of TUG1,UCA1,CEA and CYFRA21-1 alone,and it is expected to become a potential diagnostic biomarker for the diagnosis of NSCLC.
keywords: Non-small cell lung cancer Plasma Long non-coding RNA Taurine up-regulated gene 1 Urothelial carcinoma associated antigen 1
文章编号： 中图分类号：R734.2 文献标志码：A
基金项目:江苏大学附属医院科研项目(jdfyRc-2015003)
| Author Name | Affiliation |
| TANG Ling-ling,CHEN Ping,ZHOU Tian-lei,LI Jian | Department of Respiratory Medicine,Affiliated Hospital of Jiangsu University,Zhenjiang,Jiangsu 212001,Chin |
引用文本：
唐玲玲,陈萍,周天磊,李坚.非小细胞肺癌患者血浆lncRNATUG1与lncRNAUCA1的[J].中国临床研究,2021,34(1):7-12.
唐玲玲,陈萍,周天磊,李坚.非小细胞肺癌患者血浆lncRNATUG1与lncRNAUCA1的[J].中国临床研究,2021,34(1):7-12.