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投稿时间：2020-02-12 网络发布日期：2020-09-20
投稿时间：2020-02-12 网络发布日期：2020-09-20
中文摘要: 目的 探究基质金属蛋白酶(MMP)-9、单核细胞趋化蛋白-1(MCP-1)、P-选择素（CD62P）在慢性肾脏病(CKD)患者血清中的表达及意义。方法 选取2017年2月至2019年9月收治的CKD患者71例,根据患者CKD严重程度分为CKD3组16例,CKD4组18例,CKD5组37例,随机选取同期进行健康体检者20例作为对照组。使用酶联免疫吸附试验法检测MMP-9、MCP-1水平,使用流式细胞仪检测CD62P水平。MMP-9、MCP-1、CD62P在CKD的相关性,MMP-9、MCP-1、CD62P水平与CKD相关性。结果 CKD3组、CKD4组、CKD5组MMP-9、MCP-1、CD62P水平高于对照组,CKD4组、CKD5组高于CKD3组,CKD5组高于CKD4组,差异有统计学意义(P<0.05)。MMP-9、MCP-1之间,MMP-9、CD62P之间,MCP-1、CD62P之间均呈正相关(P<0.05,P<0.01)。MMP-9、MCP-1、CD62P水平与CKD严重程度具有一定相关性。结论 MMP-9、MCP-1、CD62P在CKD中表达异常,并且三者之间具有一定相关性,对MMP-9、MCP-1、CD62P表达水平进行检测,能够为CKD患者疾病严重程度的临床诊断提供一定的帮助。
中文关键词: 慢性肾脏病 基质金属蛋白酶-9 单核细胞趋化蛋白-1 P-选择素
Abstract:Objective To investigate the expression and significance of matrix metalloproteinase-9 (MMP-9), monocyte chemoattractant protein-1 (MCP-1) and P-selectin(CD62P) in serum of patients with chronic kidney disease (CKD). Methods A total of 71 patients with CKD from February 2017 to September 2019 were selected and divided into CKD3 group (16 cases), CKD4 group (18 cases) and CKD5 group (37 cases) according to the severity of CKD, and 20 cases of healthy people were selected as the control group at the same period.The levels of MMP-9 and MCP-1 were detected by enzyme-linked immunosorbent assay (ELISA), and the levels of CD62P were detected by flow cytometry.The levels of MMP-9, MCP-1 and CD62P were correlated with CKD. Results The levels of MMP-9, MCP-1 and CD62P in CKD3 group, CKD4 group and CKD5 group were higher than those in control group, CKD4 group and CKD5 group were higher than CKD3 group, CKD5 group was higher than CKD4 group, the difference was statistically significant (P<0.05).MMP-9 and MCP-1, MMP-9 and CD62P, MCP-1 and CD62P were positively correlated (P<0.05, P<0.01).The levels of MMP-9, MCP-1 and CD62P were correlated with the severity of CKD. Conclusion The expressions of MMP-9, MCP-1 and CD62P were abnormal in CKD, and there was a certain correlation among them.The detection of MMP-9, MCP-1 and CD62P expression levels can provide some help for the clinical diagnosis of CKD patients.
keywords: Chronic kidney disease Matrix metalloproteinase-9 Monocyte chemoattractant protein-1 P-selectin
文章编号： 中图分类号： 文献标志码：B
基金项目:河北省卫生和计划生育委员会科研基金项目(20191701)
| Author Name | Affiliation |
| LIU Jing*, PING Li-ying | *Department of Nephrology, Xingtai People′s Hospital, Xingtai, Hebei 054000, China |
引用文本：
刘静,平立英.MMP-9、MCP-1、CD62P在慢性肾脏病患者血清中的表达[J].中国临床研究,2020,33(9):1217-1220.
刘静,平立英.MMP-9、MCP-1、CD62P在慢性肾脏病患者血清中的表达[J].中国临床研究,2020,33(9):1217-1220.