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投稿时间：2019-04-01 网络发布日期：2020-01-20
投稿时间：2019-04-01 网络发布日期：2020-01-20
中文摘要: 目的 探讨类泛素样含PHD和环指域蛋白(UHRF)1、UHRF2蛋白在结直肠癌组织中的表达,并分析其对结直肠癌患者预后的判断价值。
方法 采用组织芯片联合免疫组化染色技术,检测2009年1月至2014年9月手术治疗患者294例结直肠癌组织及其中254例配对癌旁正常组织中UHRF1及UHRF2蛋白的表达情况,分析两者与结直肠癌发生发展的相关性及其与结直肠癌患者临床病理指标和预后的关系。
结果 免疫组织化学结果显示,UHRF1、UHRF2阳性表达定位于细胞核,UHRF1、UHRF2蛋白阳性表达率在结直肠癌组织中均显著高于癌旁正常组织(49.6% vs 3.5%,P=0.000;60.2% vs 3.1%,P=0.000),且两者呈正相关(r=0.358,P=0.008);两种蛋白的表达与肿瘤的分化程度、淋巴结转移及临床分期密切相关(P＜0.05,P＜0.01),而与患者年龄、性别、肿瘤位置、肿瘤类型、肿瘤大小无关(P均＞0.05);Kaplan-Meier生存分析显示,UHRF1、UHRF2阳性表达患者术后生存率低于阴性表达患者,两者均阳性表达患者术后生存率更低;Cox回归分析表明,UHRF1及UHRF2均为结直肠癌患者术后预后的独立影响因素(RR=3.436,P=0.016;RR=3.499,P=0.033)。
结论 UHRF1、UHRF2与结直肠癌的发生发展密切相关,二者的表达可做为患者预后判断的重要依据。
中文关键词: 结直肠癌 类泛素样含PHD和环指域蛋白1 类泛素样含PHD和环指域蛋白2 组织芯片 免疫组化
Abstract:Objective To explore the significance of the expression of UHRF1 and UHRF2 proteins in colorectal cancer tissues,and to analyze their value in judging the prognosis of colorectal cancer patients.
Methods Tissue chip combined with immunohistochemical staining were used to detect the expression of UHRF1 and UHRF2 proteins in 294 colorectal cancer tissues and 254 matched paracancerous normal tissues.The correlation between UHRF1 and UHRF2 proteins and the occurrence and development of colorectal cancer,as well as their relationship with the clinicopathological indexes and prognosis of colorectal cancer patients were analyzed.
Results The results of immunohistochemistry showed that the positive expression of UHRF1 and UHRF2 were localized in the nucleus.The positive expression rate of UHRF1 and UHRF2 in colorectal cancer was significantly higher than those in normal tissues (49.6% vs 3.5%,P=0.000;60.2% vs 3.1%, P=0.000), and there was a positive correlation between them (r=0.358,P=0.008). The expression of the two proteins was closely correlated to the degree of tumor differentiation, lymph node metastasis and clinical stage (P<0.05, P<0.01), but not to the patient's age, sex, tumor location, tumor type and tumor size (all P>0.05). Kaplan-Meier survival analysis showed that the postoperative survival rate of patients with positive expression of UHRF1 and UHRF2 was lower than those of patients with negative expression, and patients with both positive expressions had lower postoperative survival rates. Cox regression analysis showed that UHRF1 and UHRF2 were independent factors influencing the prognosis of patients with colorectal cancer (RR=3.436,P=0.016;RR=3.499,P=0.033).
Conclusion UHRF1 and UHRF2 are closely related to the occurrence and development of colorectal cancer,and their expressions can be used as an important basis for judging the prognosis of patients.
keywords: Colorectal cancer Ubiquitin-like PHD and ring finger domains 1 Ubiquitin-like PHD and ring finger domains 2 Tissue chip Immunohistochemistry
文章编号： 中图分类号： 文献标志码：A
基金项目:辽宁省科学技术计划项目(2015020377)
| Author Name | Affiliation |
| JING Hao-yue,ZENG Xian-dong,LIU Lin,SONG Zhi | Department of General Surgery，Affiliated Central Hospital of Shenyang Medical College,Shengyang ,Liaoning 110034,China |
引用文本：
景皓月,曾宪东,刘琳,宋治.UHRF1、UHRF2蛋白表达与结直肠癌关系的研究[J].中国临床研究,2020,33(1):1-5.
景皓月,曾宪东,刘琳,宋治.UHRF1、UHRF2蛋白表达与结直肠癌关系的研究[J].中国临床研究,2020,33(1):1-5.