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投稿时间：2019-01-05 网络发布日期：2019-09-24
投稿时间：2019-01-05 网络发布日期：2019-09-24
中文摘要: 目的探讨益气通络抗癌方联合替吉奥含药血清作用于体外培养的人胃癌SGC-7901细胞，对其增殖和细胞周期的影响。方法应用中药血清药理学方法制备替吉奥(替吉奥组)、益气通络抗癌方联合替吉奥(中药联合替吉奥组)及0.9%氯化钠溶液(生理盐水组)含药血清;CCK-8 法检测各组含药血清作用24、48、72 h后对SGC-7901细胞增殖的影响;流式细胞仪检测各组含药血清干预24、48、72 h后对SGC-7901细胞周期的影响;Western blot检测24、48、72 h细胞周期蛋白(cyclin)A、cyclin D1、cyclin E及细胞周期蛋白依赖性激酶（CDK2）蛋白的表达。结果除生理盐水组外,各组含药血清对SGC-7901细胞均有抑制增殖作用,中药联合替吉奥组抑制率高于替吉奥组(P<0.05);与生理盐水组比较,各组含药血清SGC-7901细胞G2/M期细胞比例均有不同程度减少;中药与替吉奥合用能够减少SGC-7901 细胞中cyclin A的表达(P<0.05),使用72 h时可增加CDK2的表达(P<0.05)；中药联合替吉奥组cyclin D1及cyclin E蛋白的表达量在24、48、72 h与生理盐水组相比差异无统计学意义(P>0.05)。24、72 h时中药联合替吉奥组cyclin D1表达量低于替吉奥组(P<0.05)。结论益气通络抗癌方联合替吉奥可有效抑制胃癌SGC-7901细胞的增殖,抑制其S/G2期的转化,下调蛋白Cyclin A表达可能是其对SGC-7901细胞发挥S/G2期转化抑制作用的机制之一。
Abstract:Objective To investigate the effect of drug-containing serum of Yiqi Tongluo Anti-cancer decoction combined with tegafur-gimeracil-oteracil potassium (TS-1) on proliferation and cell cycle of human gastric cancer SGC-7901 cells. Methods According to serum pharmacological method of Chinese medicine,the serum containing TS-1 (TS-1 group),serum containing Yiqi Tongluo Anti-cancer decoction combined with TS-1 (Chinese medicine combined with TS-1 group) and serum containing 0.9% sodium chloride solution (saline group) were prepared.CCK-8 was used to detect the effects of drug-containing serum on the proliferation of SGC-7901 cells at 24、48、72 h after treatment;flow cytometry was used to detect the effects of drug-containing serum on the cell cycle of SGC-7901 at 24、48、72 h after treatment;Western blot was used to detect the expression of cyclin A,cyclin D1,cyclin E and CDK2 protein at 24、48、72 h after treatment. Results Except for saline group,the drug-containing serum of each group inhibited the proliferation of SGC-7901 cells,and the inhibitory effect of Chinese medicine combined with TS-1 group was better than that of TS-1 group (all P<0.05).Compared with saline group,the proportion of G2/M phase cells of SGC-7901 cells in each group decreased in varying degrees.Combination of Chinese medicine and TS-1 reduced the expression of cyclin A in SGC-7901 cells (P<0.05),and increased expression of CDK2 after 72 h (P<0.05),but there was no significant difference in the expression of cylin D1 and cyclin E between Chinese medicine combined with TS-1 group and saline group after 24、48、72 h(all P>0.05).At 24 h and 72 h after treatment,the expression of cyclin D1 in Chinese medicine combined with TS-1 group was significantly lower than that in TS-1 group (P<0.05). Conclusion Yiqi Tongluo Anti-cancer decoction combined with TS-1 can effectively inhibit the proliferation and S/G2 phase transformation of SGC-7901 cells.Downregulating the expression of cyclin A may be one of the mechanisms of its inhibition on S/G2 phase transformation of SGC-7901 cells.
keywords: Yiqi Tongluo Anti-cancer decoction Tegafur-gimeracil-oteracil potassium Drug-containing serum Gastric cancer SGC-7901 Proliferation Cell cycle
文章编号： 中图分类号：R 735.2 文献标志码：B
基金项目:
| Author Name | Affiliation |
| LIU Xu,CHEN Su-ning | Department of Chinese Medicine,Shengjing Hospital of China Medical University,Shenyang,Liaoning 110004,China |
引用文本：
柳旭,陈苏宁.益气通络抗癌方联合替吉奥含药血清对人胃癌SGC-7901细胞增殖和细胞周期的影响[J].中国临床研究,2019,32(9):1265-1270.
柳旭,陈苏宁.益气通络抗癌方联合替吉奥含药血清对人胃癌SGC-7901细胞增殖和细胞周期的影响[J].中国临床研究,2019,32(9):1265-1270.